Under proper conditions, fluorescein-based dyes (e.g., the standard 6-FAM dye used for the probes in our kits) have excellent signal intensity. However, they are susceptible to a relatively high rate of photobleaching and are sensitive to acidic environments (ideal pH is 7.2 or higher), which can diminish the signal over time.

To minimize photobleaching, we recommend using a maximally sensitive detection system with the lowest possible intensity of excitation.